内容标题21

  • <tr id='Ah227p'><strong id='Ah227p'></strong><small id='Ah227p'></small><button id='Ah227p'></button><li id='Ah227p'><noscript id='Ah227p'><big id='Ah227p'></big><dt id='Ah227p'></dt></noscript></li></tr><ol id='Ah227p'><option id='Ah227p'><table id='Ah227p'><blockquote id='Ah227p'><tbody id='Ah227p'></tbody></blockquote></table></option></ol><u id='Ah227p'></u><kbd id='Ah227p'><kbd id='Ah227p'></kbd></kbd>

    <code id='Ah227p'><strong id='Ah227p'></strong></code>

    <fieldset id='Ah227p'></fieldset>
          <span id='Ah227p'></span>

              <ins id='Ah227p'></ins>
              <acronym id='Ah227p'><em id='Ah227p'></em><td id='Ah227p'><div id='Ah227p'></div></td></acronym><address id='Ah227p'><big id='Ah227p'><big id='Ah227p'></big><legend id='Ah227p'></legend></big></address>

              <i id='Ah227p'><div id='Ah227p'><ins id='Ah227p'></ins></div></i>
              <i id='Ah227p'></i>
            1. <dl id='Ah227p'></dl>
              1. <blockquote id='Ah227p'><q id='Ah227p'><noscript id='Ah227p'></noscript><dt id='Ah227p'></dt></q></blockquote><noframes id='Ah227p'><i id='Ah227p'></i>

                绍兴市创烨生物科技有限公司www.cy-bio.com To order:0575-82578768
                Fax:0575-82578758
                Email:Sales@cy-bio.com
                首页 公司简?/strong> 新闻资讯 产品展示 有问必答 人才招聘 联系我们


                 产品目录
                科研检测试?/strong>
                  分光光Цf法科研检测试?/font>
                可见紫外分光光度计检测系?/font>
                半自动生化分ء析仪检测系?/font>
                全自动生化分析仪检测系?/font>
                  酶标仪法科研检测试?/font>
                  校准品、质控品系列
                校准品系?/font>
                质控品系?/font>
                体外诊断试剂技术支?/strong>
                  肝功能系?/font>
                  血脂、冠心病系列
                  肾功能e?/font>
                  糖代谢系?/font>
                  离子系列
                  心肌功能糡列
                  风湿系列
                  胰腺疨病系列
                  特种蛋白系列
                  其他
                科研及衾断试剂原材料
                  显色底物
                  显色色源
                  生物缹_ڵ冲?/font>
                  分子生物学试?/font>
                  医药中间体系列及其他
                  科研及诊断用?/font>
                  牛血清、牛血清白蛋白、抗血?/font>
                  进口常用生化试剂原料Ȼh目录
                 您的位置:首?nbsp;>> 新闻资讯
                【特别推荐】:高密度脂蛋白3-胆̛醇(HDL3-C)测定试?/strong>  
                发布者:idrfz.chen   
                 

                (本试剂仅用于科研、实验、技术支持,不直接应用于临床诊断?/span>

                【简介?/span>

                用于全自动生化分析仪测定高密度脂蛋白3-胆固醇含量?/span>

                高密度脂蛋白胆固醇(HDL-C)是冠心病(CHD)的负面危险因素?/span>HDL-C可以进一步分为两大类,更大、更轡的HDL2-C颗粒和更小、更致密?/span>HDL3-C颗粒。已有的临床数据表明,测?/span>HDL3-C比涭ҲЦu˓u^量?/span>HDL-C更能反映CHD风险?/span>

                【测定方法?/span>

                匀相直接法

                【测定原理?/span>

                第一步,试剂Ⅰ与样本充分混合并孵?-5分钟。第二步, HDL3-C颗粒被裂解,并参?/span>Trinder’S反应后显色?/span>

                【试剂盒组成?/span>

                 

                ?/span>    ?/span>

                ?/span>    ?/span>

                ?/span>    ?/span>

                试剂

                液体双试?/span>

                试剂Ⅰ:试剂?/span>=3?/span>1

                4-氨基安替比林?/span>TOOS、工具酶?/span>

                适量

                HDL3-C校准?/span>

                冻干1ml

                HDL3-C?/span>

                见标?/span>

                【样本要求?/span>

                标本应空腹抽取,尽快分离血清,在冷藏(2-8?/span>)条件可稳定3天。标本如Ψ֑{ճF能及时测定,应?/span>-80℃ҲȺô̽翝存,叢ٯ稳?/span>7个月,避免反复冻融?/span>

                【测定步骤?/span>

                1.本试剂为液体双试剂,可直接使用?/span>

                2.测定参数:波长:600nm;光径:10mm;温度:37?/span>?/span>

                3.测定斡法?/span>

                加入?/span>

                空白?/span>

                标准?/span>

                测定?/span>

                试剂?/span>

                225

                225

                225

                蒸馏?/span>(uL)

                3

                -

                -

                标准?/span>(uL)

                -

                3

                -

                样本(uL)

                -

                -

                3

                混匀,置37?/span>孵育3-5分钟,读取各管吸光度A1

                试剂?/span>(uL)

                75

                75

                75

                混匀,置37?/span>保温5分o璟,读取各管吸光度A2,计算?/span>A=A2-A1

                4.上机Ҫ㳼㲻参数?/span>

                ?/span>  ?/span>

                37?/span>

                血?/span>+R1反应时间

                300?/span>

                样本?/span>

                3uL

                ?/span> ?/span> ?/span>

                600nm

                试剂?/span>

                225uL

                ?/span> ?/span> ?/span>

                700nm

                试剂?/span>

                75uL

                血?/span>+R1+R2反应时间

                300?/span>

                5.计算?/span>

                HDL3-C含量 ?/span>mmol/L?/span>=  [(?/span>A标本-ΔA空白?/span>(?/span>A标准-ΔA空白?/span>]  × 校准品?/span>

                【性能指标?/span>

                1?/span> 空白吸光?/span> ?/span>0.05?/span>

                2?/span> 线性范?/span>0-2.60mmol/L?/span>1004mg/L);

                3?/span> 批内/分析内精密度  CV?/span>5%;批间相对极差 ?/span>7%;

                4?/span> 准确?/span>  相对偏差不超过?/span>10%?/span>

                5?/span> 抗干扰?/span> 血清胆红素<40 mg/dL?/span>TG<1000mg/dL、血红蛋?/span><500 mg/dL、抗坏血?/span><10mmol/L时,对测定无显著影响?/span>

                6?/span> 方法对照 用本测定ԓ͎prڲ试剂和某进口HDL3-C测定试剂~t͉Ķī뿴^ȥ测定100例血?/span>HDL3-C含量Anj结果显示相关系?/span>>0.960?/span>

                【注意事项?/span>

                1、试剂变混浊或空白吸光度值L0.05时,将不能使用,ˮذӭρ弃去;

                2、建议各实验室建立自己的正常值范围Ǿf

                3、试剂与样本量可根据需要按比˼调节?/span>

                4、请勿用嘴直接吸取试剂,避免接触皮肤、眼睛及粘膜,一旦接触,应即用水冲洗污染部位?/span>

                5、使用配套的校准品校准,校准周期?/span>30天,更试剂批号时需要重新校准。建议使用正常值和病理值生化质控血清进行室内质控,测定的控制值应在确定的限制范围ֹһЦ内,若控制值失控,实验˶X室应采取适当的纠正措施?/span>

                6、不能将R1?/span>R2混成单试剂工作液?/span>

                【储存条件与有效期?/span>

                未开封的试剂?/span>2-8?/span>避光条件下可稳定14个月,置37?/span>水浴至少可稳?/span>7天?/span>

                试剂开封后,存放在分析仪试剂仓?/span>(2-8?/span>)的试剂塼30天内性质稳定?/span>

                【参考文献?/span>

                1. Stampfer MJ et al. N Engl J Med 1991; 325: 373-81.

                2. Williams PT. J Lipid Res 2012; 53: 266-72.

                3. Mueller O et al. Clin Chem Lab Med 2008; 46: 490-8.

                 


                首页  | 公司简?/font>  | 新闻资讯  | 产品展示  | 有问必答  | 人才招聘  | 联系我们
                绍兴市创烨生物科技有限公司
                电话?575-82578768    传真?575-82578758    邮箱:sales@cy-bio.com 备案号: ĘҲ浙ICP?9014690?1
                地址:浙江省绍兴市上虞区高新技术产业园区鸿天工业园     网址Awww.cy-bio.com    ©All Rights Reserved  技术支?致梦大数?